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Price
Variant Calling $20/Sample

DNA Extraction
$20/Sample

Short read sequencing, Illumina

Sample requirements: >10ng/ul in at least 30ul of volume.

Our short read sequencing service provides 150bp paired end reads. Short reads can be useful for a range of applications, including genotyping of clones, whole population and metagenomic sequencing, and amplicon/microbiome profiling. Quantities listed are guaranteed minimums.

Price

Qty

Variant Calling
$20/sample

Our variant calling service includes mapping of short reads against a reference genome. Our service builds upon the best-of-class breseq software to provide intuitive insights into the genetic differences between your sample and the genome reference.

DNA Extraction
$15/sample

Small; 200 Mbp/1.3 Million reads

From: $0.00

  • Short reads; small 200 Mbp/1.3 Million reads

    $77.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each
SKU: 1201 Category:

Additional information

Short reads; small 200 Mbp/1.3 Million reads

TYPE

Small, DNA, Variant

Medium; 400 Mbp/2.7 Million reads

From: $0.00

  • Short reads; medium 400 Mbp/2.7 Million reads

    $101.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each
SKU: 1401 Category:

Medium-large; 650 Mbp/4.3 Million reads

From: $0.00

  • Short reads; medium-large 650 Mbp/4.3 Million reads

    $125.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each
SKU: 1501 Category:

Large; 1000 Mbp/6.7 Million reads

From: $0.00

  • Short reads; large 1000 Mbp/6.7 Million reads

    $139.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each
SKU: 1601 Category:

XL 2000Mbp/13.3 Million reads

From: $0.00

  • Short reads; XL 2000Mbp/13.3 Million reads

    $192.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each
SKU: 1631 Category:

Additional information

Short reads; XL 2000Mbp/13.3 Million reads

TYPE

Small, DNA, Variant

XXL 5000Mbp/33.3 Million reads

From: $0.00

  • Short reads; XXL 5000Mbp/33.3 Million reads

    $384.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each
SKU: 1641 Category:

Additional information

Short reads; XXL 5000Mbp/33.3 Million reads

TYPE

Small, DNA, Variant

XXXL 10000Mbp/66.7 Million reads

From: $0.00

  • Short reads; XXXL 10000Mbp/66.7 Million reads

    $528.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each
SKU: 1651 Category:

Additional information

Short reads; XXXL 10000Mbp/66.7 Million reads

TYPE

Small, DNA, Variant

custom sequencing services-Illumina

From: $0.00

  • Short reads; custom sequencing services

    $0.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each
SKU: 1691 Category:

Long read sequencing, Oxford Nanopore

Sample requirements: >40ng/ul in at least 60ul of volume.

Our long read sequencing service provides reads up to 100kbp (occasionally much longer) in length. To maximize read length we strongly recommend using our extraction service, because long DNA fragments may shear during shipping and with some extraction methods.

Long reads can be useful for a range of applications, including sequencing longer amplicons, repetitive plasmids or viruses, spanning difficult to map/assemble regions, and for resolving complex metagenomes. Quantities listed are guaranteed minimums.

Price

Qty

Variant Calling
$20/sample

DNA Extraction
$15/sample

Small; 300 Mbp/300k reads

From: $0.00

  • Long reads; small 300 Mbp/300k reads

    $255.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each

medium 600 Mbp/600k reads

From: $0.00

  • Long reads; medium 600 Mbp/600k reads

    $325.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each

Large; 900 Mbp/900k reads

From: $0.00

  • Long reads; large 900 Mbp/900k reads

    $366.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each

XL 1500 Mbp/1500k reads

From: $0.00

  • Long reads; XL 1500 Mbp/1500k reads

    $450.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each

custom sequencing services-Nanopore

From: $0.00

  • Long reads; custom sequencing services

    $0.00 each
  • Variant Calling Standard

    $20.00 each
  • DNA Extraction

    $15.00 each

Our convenient and  comprehensive strain typing service bundles bead-beating extraction, short read sequencing, and bioinformatics analysis to produce reports on the MLST (multi-locus sequence type) of your strain of interest.

Please send frozen cell pellets (50-100mg) or sealed, pre-grown agar plates.

Price

Qty

ST 200 Mbp/1.3 Million reads and report

From: $0.00

  • Strain Typing; small 200 Mbp/1.3 Million reads and report

    $168.00 each
SKU: 7151 Category:

ST custom services and reports

From: $0.00

  • Strain Typing; custom services and reports

    $0.00 each
SKU: 7191 Category:

  Please contact us for pricing of long read Nanopore strain typing

Sample requirements: >10ng/ul in at least 30ul of water, samples must be free of PCR inhibitors including EDTA.

Use full-length 16S sequencing to maximize species and strain-level resolution. Custom pricing for full-length 16S starts at 24 samples; contact us for more information.

Quantities listed are guaranteed minimums.

Contact us for pricing information on analysis to get community composition and diversity metrics, in both table and graph formats.

Price

Qty

DNA Extraction
$15/sample

V3/V4 20k reads, 2x300bp

From: $0.00

  • 16S V3/V4 20k reads, 2x300bp

    $43.00 each
  • DNA Extraction

    $15.00 each
SKU: 6111 Category:

V3/V4 50k reads, 2x300bp

From: $0.00

  • 16S V3/V4 50k reads, 2x300bp

    $53.00 each
  • DNA Extraction

    $15.00 each
SKU: 6121 Category:

V3/V4 100k reads, 2x300bp

From: $0.00

  • 16S V3/V4 100k reads, 2x300bp

    $73.00 each
  • DNA Extraction

    $15.00 each
SKU: 6131 Category:

full-length 10k reads, Nanopore

From: $0.00

  • 16S full-length 10k reads, Nanopore

    $74.00 each
  • DNA Extraction

    $15.00 each
SKU: 6311 Category:

full-length 25k reads, Nanopore

From: $0.00

  • 16S full-length 25k reads, Nanopore

    $102.00 each
  • DNA Extraction

    $15.00 each
SKU: 6321 Category:

full-length; 50k reads, Nanopore

From: $0.00

  • 16S full-length; 50k reads, Nanopore

    $123.00 each
  • DNA Extraction

    $15.00 each
SKU: 6331 Category:

  Please contact us for custom primers, including ITS, and for analysis pricing

Hybrid whole genome sequencing
-bundled extraction and sequencing

Sample requirements: frozen cell pellet (50-100mg) or sealed, pre-grown agar plate.

Our hybrid sequencing service provides both 150bp paired end reads in combination with long reads for a given sample. HMW DNA extraction is also included!

This sequencing strategy is especially useful for building robust reference genomes; the long reads drastically reduce the number of contigs and the depth afforded by the short reads increases the confidence of each nucleotide position.

Price

Qty

small 400+300 Mbp/3 Million reads

From: $0.00

  • Hybrid; small 400+300 Mbp/3 Million reads (up to 8Mbp genomes)

    $357.00 each

medium 1000+600 Mbp/7.3 Million reads

From: $0.00

  • Hybrid; medium 1000+600 Mbp/7.3 Million reads (up to 20Mbp genomes)

    $460.00 each

large 2000+1200 Mbp/14.5 Million reads

From: $0.00

  • Hybrid; large 2000+1200 Mbp/14.5 Million reads (up to 40Mbp genomes)

    $589.00 each

  Please contact us for analysis pricing

Poly-A Enrichment RNAseq, Illumina
-compatible with eukaryotes only

Our RNA sequencing service provides 150bp paired end reads.

Please send >50ng/ul of RNA in a volume of at least 20ul (more is better!), shipped frozen on dry ice. We recommend that sample concentrations be quantified by Qubit or other fluorometry-based techniques, as Nanodrop methods tend to overestimate quantity. DNAse treatment during extraction is recommended. For optimal results, the RIN should be at least 7.

Price

Qty

polyA; 12M reads, 2x150bp

From: $0.00

  • RNAseq polyA; 12M reads, 2x150bp

    $240.00 each
SKU: 1851 Category:

polyA; 25M reads, 2x150bp

From: $0.00

  • RNAseq polyA; 25M reads, 2x150bp

    $288.00 each
SKU: 1861 Category:

polyA; 50M reads, 2x150bp

From: $0.00

  • RNAseq polyA; 50M reads, 2x150bp

    $394.00 each
SKU: 1871 Category:

custom RNAseq services

From: $0.00

  • RNAseq; custom RNAseq services

    $0.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 1891 Category:

Ribodepletion RNAseq, Illumina

Our RNA sequencing service provides 150bp paired end reads.

Please send >50ng/ul of RNA in a volume of at least 20ul (more is better!), shipped frozen on dry ice. We recommend that sample concentrations be quantified by Qubit or other fluorometry-based techniques, as Nanodrop methods tend to overestimate quantity. DNAse treatment during extraction is recommended. For optimal results, the RIN should be at least 7.

Price

Qty

-compatible with eukarotes and prokaryotes

12M reads, 2x150bp

From: $0.00

  • RNAseq ribo; 12M reads, 2x150bp

    $259.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 1811 Category:

25M reads, 2x150bp

From: $0.00

  • RNAseq ribo; 25M reads, 2x150bp

    $312.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 1821 Category:

50M reads, 2x150bp

From: $0.00

  • RNAseq ribo; 50M reads, 2x150bp

    $413.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 1831 Category:

custom RNAseq services

From: $0.00

  • RNAseq; custom RNAseq services

    $0.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 1891 Category:

Bioinformatics

Price

Qty

  Variant Calling Standard – ordered via bundle above.

VC Deluxe

From: $0.00

  • Variant Calling Deluxe

    $40.00 each
SKU: 8151 Category:

Prokaryotes

From: $0.00

  • Genome assembly; Prokaryotes

    $50.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 8211 Category:

Eukaryotes

From: $0.00

  • Genome assembly; Eukaryotes

    $100.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 8221 Category:

Differential expression

From: $0.00

  • Differential expression analysis

    $75.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 8281 Category:

Additional information

Differential expression analysis

TYPE

Small, DNA, Variant

Annotation-paired-product

From: $0.00

  • Annotation

    $100.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 8411 Category:

Additional information

Annotation

TYPE

Small, DNA, Variant

Amplicon

From: $0.00

  • Amplicon/microbiome analysis

    $35.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 8251 Category:

Additional information

Amplicon/microbiome analysis

TYPE

Small, DNA, Variant

Transcriptome

From: $0.00

  • Transcriptome assembly

    $100.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 8261 Category:

Additional information

Transcriptome assembly

TYPE

Small, DNA, Variant

Metagenome

From: $0.00

  • Metagenome assembly

    $100.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 8511 Category:

Additional information

Metagenome assembly

TYPE

Small, DNA, Variant

Pangenomic

From: $0.00

  • Pangenomic analysis

    $25.00 each
  • Spacer

    $0.00 each
  • Spacer

    $0.00 each
SKU: 8611 Category:

Additional information

Pangenomic analysis

TYPE

Small, DNA, Variant

binning and bin curation

From: $0.00

  • Metagenome binning and bin curation

    $250.00 each
SKU: 8521 Category:

Additional information

Metagenome binning and bin curation

TYPE

Small, DNA, Variant

classification and phylogenomics

From: $0.00

  • Taxonomic classification and phylogenomics

    $150.00 each
SKU: 8531 Category:

Additional information

Taxonomic classification and phylogenomics

TYPE

Small, DNA, Variant

  Please contact us for custom bioinformatics services

Prepaid Sequencing and Bioinformatics

Price

Qty

valid for one year from purchase date

From: $0.00

  • Insights Vouchers (valid for one year from purchase date)

    $100.00 each
SKU: 1011 Category:
Have a large project? We offer tailored quotes  and attractive discounts on orders of 24+ samples or bulk sequencing orders, such as full flow cells and substantial data volumes.
 Contact us to obtain a personalized quote that’s adjusted to your project’s size and timeline.

Please click the “Add to Cart” button once – only once.

Sample Preparation Guidelines

Nucleic Acid Samples

Service Concentration* Volume Buffer Shipping Considerations
Short read sequencing, Illumina
>10 ng/µL
>30 µL
nuclease-free water or dilute TE (1:10)
Can be shipped at room temperature.
Long read sequencing, Oxford Nanopore
>50 ng/µL
>60 µL
nuclease-free water
Should be shipped with ice packs or on dry ice.
Poly-A Enrichment RNAseq, Illumina
>25 ng/µL RNA
>20 µL. Please include a second aliquot (~5 µL) for sample QC.
nuclease-free water or dilute TE (1:10)
RNA should be shipped overnight on dry ice.
Ribodepletion RNAseq, Illumina
>25 ng/µL RNA
>20 µL. Please include a second aliquot (~5 µL) for sample QC.
nuclease-free water or dilute TE (1:10)
RNA should be shipped overnight on dry ice.
16S Amplicon (Illumina or Nanopore)
>10 ng/µL
>30 µL
nuclease-free water
Can be shipped at room temperature.
Full Flow Cell Runs, Illumina
Prepared and pooled libraries with a final concentration of at least 2 nM.
RBS + Tween
Can be shipped with ice packs or on dry ice.
Full Flow Cell Runs, Nanopore
Same as long read Nanopore
nuclease-free water
Should be shipped with ice packs or on dry ice.

*We recommend quantifying by Qubit, as Nanodrop can overestimate concentrations >10-fold if contaminants are present.

We highly recommend eluting samples in nuclease-free water or diluted TE, as excessive EDTA can interfere with with library prep. Extraction contaminants (salts, phenol, polysaccharides, etc.) can cause sample failure.

Services that include DNA Extraction

Service Sample Type Shipping Considerations
Hybrid whole genome sequencing, Illumina/Nanopore OR Strain typing, Illumina
Frozen cell pellets (50 - 100 mg), all supernatent removed; OR Sealed, pre-grown agar plates - must have enough growth to allow us to easily collect 50 - 100 mg of colonies.
Ship frozen cell pellets on dry ice. Ship agar plates with ice packs or at room temperature.

Looking for detailed guidance on selecting the right sequencing approach for your science? Our consultation services provide expert advice tailored to your project's needs.