Price
DNA Extraction
$20/Sample
Short read sequencing, Illumina
Sample requirements: >10ng/ul in at least 30ul of volume.
Our short read sequencing service provides 150bp paired end reads. Short reads can be useful for a range of applications, including genotyping of clones, whole population and metagenomic sequencing, and amplicon/microbiome profiling. Quantities listed are guaranteed minimums.
Price
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Variant Calling
$20/sample
Our variant calling service includes mapping of short reads against a reference genome. Our service builds upon the best-of-class breseq software to provide intuitive insights into the genetic differences between your sample and the genome reference.
DNA Extraction
$15/sample
Small; 200 Mbp/1.3 Million reads
From: $0.00
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Short reads; small 200 Mbp/1.3 Million reads
| TYPE | Small, DNA, Variant |
|---|
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Medium; 400 Mbp/2.7 Million reads
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Medium-large; 650 Mbp/4.3 Million reads
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Large; 1000 Mbp/6.7 Million reads
From: $0.00
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XL 2000Mbp/13.3 Million reads
From: $0.00
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Short reads; XL 2000Mbp/13.3 Million reads
| TYPE | Small, DNA, Variant |
|---|
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XXL 5000Mbp/33.3 Million reads
From: $0.00
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Short reads; XXL 5000Mbp/33.3 Million reads
| TYPE | Small, DNA, Variant |
|---|
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XXXL 10000Mbp/66.7 Million reads
From: $0.00
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Short reads; XXXL 10000Mbp/66.7 Million reads
| TYPE | Small, DNA, Variant |
|---|
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custom sequencing services-Illumina
From: $0.00
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Long read sequencing, Oxford Nanopore
Sample requirements: >40ng/ul in at least 60ul of volume.
Our long read sequencing service provides reads up to 100kbp (occasionally much longer) in length. To maximize read length we strongly recommend using our extraction service, because long DNA fragments may shear during shipping and with some extraction methods.
Long reads can be useful for a range of applications, including sequencing longer amplicons, repetitive plasmids or viruses, spanning difficult to map/assemble regions, and for resolving complex metagenomes. Quantities listed are guaranteed minimums.
Price
Qty
Variant Calling
$20/sample
DNA Extraction
$15/sample
Small; 300 Mbp/300k reads
From: $0.00
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medium 600 Mbp/600k reads
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Large; 900 Mbp/900k reads
From: $0.00
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XL 1500 Mbp/1500k reads
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custom sequencing services-Nanopore
From: $0.00
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Our convenient and comprehensive strain typing service bundles bead-beating extraction, short read sequencing, and bioinformatics analysis to produce reports on the MLST (multi-locus sequence type) of your strain of interest.
Please send frozen cell pellets (50-100mg) or sealed, pre-grown agar plates.
Price
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ST 200 Mbp/1.3 Million reads and report
From: $0.00
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ST custom services and reports
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Please contact us for pricing of long read Nanopore strain typing
Sample requirements: >10ng/ul in at least 30ul of water, samples must be free of PCR inhibitors including EDTA.
Use full-length 16S sequencing to maximize species and strain-level resolution. Custom pricing for full-length 16S starts at 24 samples; contact us for more information.
Quantities listed are guaranteed minimums.
Contact us for pricing information on analysis to get community composition and diversity metrics, in both table and graph formats.
Price
Qty
DNA Extraction
$15/sample
V3/V4 20k reads, 2x300bp
From: $0.00
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V3/V4 50k reads, 2x300bp
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V3/V4 100k reads, 2x300bp
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full-length 10k reads, Nanopore
From: $0.00
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full-length 25k reads, Nanopore
From: $0.00
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full-length; 50k reads, Nanopore
From: $0.00
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Please contact us for custom primers, including ITS, and for analysis pricing
Hybrid whole genome sequencing
-bundled extraction and sequencing
Sample requirements: frozen cell pellet (50-100mg) or sealed, pre-grown agar plate.
Our hybrid sequencing service provides both 150bp paired end reads in combination with long reads for a given sample. HMW DNA extraction is also included!
This sequencing strategy is especially useful for building robust reference genomes; the long reads drastically reduce the number of contigs and the depth afforded by the short reads increases the confidence of each nucleotide position.
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Qty
small 400+300 Mbp/3 Million reads
From: $0.00
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-
medium 1000+600 Mbp/7.3 Million reads
From: $0.00 Add to cart -
Hybrid; small 400+300 Mbp/3 Million reads (up to 8Mbp genomes)
$357.00 Add to cart -
Hybrid; large 2000+1200 Mbp/14.5 Million reads (up to 40Mbp genomes)
$589.00 Add to cart -
Hybrid; medium 1000+600 Mbp/7.3 Million reads (up to 20Mbp genomes)
$460.00 Add to cart
medium 1000+600 Mbp/7.3 Million reads
From: $0.00
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-
Hybrid; large 2000+1200 Mbp/14.5 Million reads (up to 40Mbp genomes)
$589.00 Add to cart -
small 400+300 Mbp/3 Million reads
From: $0.00 Add to cart -
Hybrid; medium 1000+600 Mbp/7.3 Million reads (up to 20Mbp genomes)
$460.00 Add to cart -
Hybrid; small 400+300 Mbp/3 Million reads (up to 8Mbp genomes)
$357.00 Add to cart
large 2000+1200 Mbp/14.5 Million reads
From: $0.00
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-
Hybrid; large 2000+1200 Mbp/14.5 Million reads (up to 40Mbp genomes)
$589.00 Add to cart -
Hybrid; medium 1000+600 Mbp/7.3 Million reads (up to 20Mbp genomes)
$460.00 Add to cart -
small 400+300 Mbp/3 Million reads
From: $0.00 Add to cart -
Hybrid; small 400+300 Mbp/3 Million reads (up to 8Mbp genomes)
$357.00 Add to cart
Please contact us for analysis pricing
Poly-A Enrichment RNAseq, Illumina
-compatible with eukaryotes only
Our RNA sequencing service provides 150bp paired end reads.
Please send >50ng/ul of RNA in a volume of at least 20ul (more is better!), shipped frozen on dry ice. We recommend that sample concentrations be quantified by Qubit or other fluorometry-based techniques, as Nanodrop methods tend to overestimate quantity. DNAse treatment during extraction is recommended. For optimal results, the RIN should be at least 7.
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Qty
polyA; 12M reads, 2x150bp
From: $0.00
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polyA; 25M reads, 2x150bp
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polyA; 50M reads, 2x150bp
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custom RNAseq services
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Ribodepletion RNAseq, Illumina
Our RNA sequencing service provides 150bp paired end reads.
Please send >50ng/ul of RNA in a volume of at least 20ul (more is better!), shipped frozen on dry ice. We recommend that sample concentrations be quantified by Qubit or other fluorometry-based techniques, as Nanodrop methods tend to overestimate quantity. DNAse treatment during extraction is recommended. For optimal results, the RIN should be at least 7.
Price
Qty
-compatible with eukarotes and prokaryotes
12M reads, 2x150bp
From: $0.00
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25M reads, 2x150bp
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50M reads, 2x150bp
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custom RNAseq services
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Bioinformatics
Price
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Variant Calling Standard – ordered via bundle above.
VC Deluxe
From: $0.00
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Eukaryotes
From: $0.00
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Differential expression
From: $0.00
Additional information
Differential expression analysis
| TYPE | Small, DNA, Variant |
|---|
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Annotation-paired-product
From: $0.00
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Annotation
| TYPE | Small, DNA, Variant |
|---|
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Amplicon
From: $0.00
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Amplicon/microbiome analysis
| TYPE | Small, DNA, Variant |
|---|
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Transcriptome
From: $0.00
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Transcriptome assembly
| TYPE | Small, DNA, Variant |
|---|
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Metagenome
From: $0.00
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Metagenome assembly
| TYPE | Small, DNA, Variant |
|---|
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Pangenomic
From: $0.00
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Pangenomic analysis
| TYPE | Small, DNA, Variant |
|---|
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binning and bin curation
From: $0.00
Additional information
Metagenome binning and bin curation
| TYPE | Small, DNA, Variant |
|---|
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classification and phylogenomics
From: $0.00
Additional information
Taxonomic classification and phylogenomics
| TYPE | Small, DNA, Variant |
|---|
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Please contact us for custom bioinformatics services
Prepaid Sequencing and Bioinformatics
Price
Qty
valid for one year from purchase date
From: $0.00
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Have a large project? We offer tailored quotes and attractive discounts on orders of 24+ samples or bulk sequencing orders, such as full flow cells and substantial data volumes.
Contact us to obtain a personalized quote that’s adjusted to your project’s size and timeline.
Sample Preparation Guidelines
Nucleic Acid Samples
| Service | Concentration* | Volume | Buffer | Shipping Considerations |
|---|---|---|---|---|
|
Short read sequencing, Illumina |
>10 ng/µL |
>30 µL |
nuclease-free water or dilute TE (1:10) |
Can be shipped at room temperature. |
|
Long read sequencing, Oxford Nanopore |
>50 ng/µL |
>60 µL |
nuclease-free water |
Should be shipped with ice packs or on dry ice. |
|
Poly-A Enrichment RNAseq, Illumina |
>25 ng/µL RNA |
>20 µL. Please include a second aliquot (~5 µL) for sample QC. |
nuclease-free water or dilute TE (1:10) |
RNA should be shipped overnight on dry ice. |
|
Ribodepletion RNAseq, Illumina |
>25 ng/µL RNA |
>20 µL. Please include a second aliquot (~5 µL) for sample QC. |
nuclease-free water or dilute TE (1:10) |
RNA should be shipped overnight on dry ice. |
|
16S Amplicon (Illumina or Nanopore) |
>10 ng/µL |
>30 µL |
nuclease-free water |
Can be shipped at room temperature. |
|
Full Flow Cell Runs, Illumina |
Prepared and pooled libraries with a final concentration of at least 2 nM. |
RSB + Tween |
Can be shipped with ice packs or on dry ice. |
|
|
Full Flow Cell Runs, Nanopore |
Same as long read Nanopore |
nuclease-free water |
Should be shipped with ice packs or on dry ice. |
|
*We recommend quantifying by Qubit, as Nanodrop can overestimate concentrations >10-fold if contaminants are present.
We highly recommend eluting samples in nuclease-free water or diluted TE, as excessive EDTA can interfere with with library prep. Extraction contaminants (salts, phenol, polysaccharides, etc.) can cause sample failure.
Services that include DNA Extraction
| Service | Sample Type | Shipping Considerations |
|---|---|---|
|
Hybrid whole genome sequencing, Illumina/Nanopore OR Strain typing, Illumina |
Frozen cell pellets (50 - 100 mg), all supernatent removed; OR Sealed, pre-grown agar plates - must have enough growth to allow us to easily collect 50 - 100 mg of colonies.* |
Ship frozen cell pellets on dry ice. Ship agar plates with ice packs or at room temperature. |
*We have found that ~10e9 E. coli cells is sufficient for our extraction workflows.
