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DNA Extraction
$20/Sample
Short read sequencing, Illumina
Sample requirements: >10ng/ul in at least 30ul of volume.
Our short read sequencing service provides 150bp paired end reads. Short reads can be useful for a range of applications, including genotyping of clones, whole population and metagenomic sequencing, and amplicon/microbiome profiling. Quantities listed are guaranteed minimums.
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Variant Calling
$20/sample
Our variant calling service includes mapping of short reads against a reference genome. Our service builds upon the best-of-class breseq software to provide intuitive insights into the genetic differences between your sample and the genome reference.
DNA Extraction
$20/sample
Small; 200 Mbp/1.3 Million reads
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Short reads; small 200 Mbp/1.3 Million reads
| TYPE | Small, DNA, Variant |
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Medium; 400 Mbp/2.7 Million reads
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Medium-large; 650 Mbp/4.3 Million reads
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Large; 1000 Mbp/6.7 Million reads
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XL 2000Mbp/13.3 Million reads
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Short reads; XL 2000Mbp/13.3 Million reads
| TYPE | Small, DNA, Variant |
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XXL 5000Mbp/33.3 Million reads
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Short reads; XXL 5000Mbp/33.3 Million reads
| TYPE | Small, DNA, Variant |
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XXXL 10000Mbp/66.7 Million reads
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Short reads; XXXL 10000Mbp/66.7 Million reads
| TYPE | Small, DNA, Variant |
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custom sequencing services-Illumina
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Long read sequencing, Oxford Nanopore
Sample requirements: >40ng/ul in at least 60ul of volume.
Our long read sequencing service provides reads up to 100kbp (occasionally much longer) in length. To maximize read length we strongly recommend using our extraction service, because long DNA fragments may shear during shipping and with some extraction methods.
Long reads can be useful for a range of applications, including sequencing longer amplicons, repetitive plasmids or viruses, spanning difficult to map/assemble regions, and for resolving complex metagenomes. Quantities listed are guaranteed minimums.
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Variant Calling
$20/sample
DNA Extraction
$20/sample
Small; 300 Mbp/300k reads
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medium 600 Mbp/600k reads
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Large; 900 Mbp/900k reads
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XL 1500 Mbp/1500k reads
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custom sequencing services-Nanopore
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Our convenient and comprehensive strain typing service bundles bead-beating extraction, short read sequencing, and bioinformatics analysis to produce reports on the MLST type of your strain of interest.
Please send frozen cell pellets (50-100mg) or sealed, pre-grown agar plates.
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ST 200 Mbp/1.3 Million reads and report
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ST custom services and reports
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Please contact us for pricing of long read Nanopore strain typing
Sample requirements: >10ng/ul in at least 30ul of water, samples must be free of PCR inhibitors including EDTA.
Use full-length 16S sequencing to maximize species and strain-level resolution. Custom pricing for full-length 16S starts at 24 samples, contact us for more information.
Quantities listed are guaranteed minimums.
The included analyses provide community composition and diversity metrics, in both table and graph formats.
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DNA Extraction
$20/sample
V3/V4 20k reads, 2x300bp
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V3/V4 50k reads, 2x300bp
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V3/V4 100k reads, 2x300bp
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full-length 10k reads, Nanopore
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full-length 25k reads, Nanopore
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full-length; 50k reads, Nanopore
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 Please contact us for custom primers, including ITS, and for analysis pricing
Hybrid whole genome sequencing
-bundled extraction and sequencing
Sample requirements: frozen cell pellet (50-100mg) or sealed, pre-grown agar plate.
Our hybrid sequencing service provides both 150bp paired end reads in combination with long reads for a given sample. HMW DNA extraction, hybrid assembly, metrics, and annotation are also included.
This sequencing strategy is especially useful for building robust reference genomes; the long reads drastically reduce the number of contigs and the depth afforded by the short reads increases the confidence of each nucleotide position.
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small 400+300 Mbp/3 Million reads
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Hybrid; medium 1000+600 Mbp/7.3 Million reads (up to 20Mbp genomes)
$460 Add to cart -
Hybrid; large 2000+1200 Mbp/14.5 Million reads (up to 40Mbp genomes)
$589 Add to cart -
medium 1000+600 Mbp/7.3 Million reads
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Hybrid; small 400+300 Mbp/3 Million reads (up to 8Mbp genomes)
$357 Add to cart
medium 1000+600 Mbp/7.3 Million reads
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large 2000+1200 Mbp/14.5 Million reads
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 Please contact us for analysis pricing
Poly-A Enrichment RNAseq, Illumina
-compatible with eukaryotes only
Our RNA sequencing service provides 150bp paired end reads.
Please send >50ng/ul of RNA in a volume of at least 20ul (more is better!), shipped frozen on dry ice. We recommend that sample concentrations be quantified by Qubit or other fluorometry-based techniques, as Nanodrop methods tend to overestimate quantity. DNAse treatment during extraction is recommended. For optimal results, the RIN should be at least 7.
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polyA; 12M reads, 2x150bp
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polyA; 25M reads, 2x150bp
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polyA; 50M reads, 2x150bp
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custom RNAseq services
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Ribodepletion RNAseq, Illumina
-compatible with eukarotes and prokaryotes
Our RNA sequencing service provides 150bp paired end reads.
Please send >50ng/ul of RNA in a volume of at least 20ul (more is better!), shipped frozen on dry ice. We recommend that sample concentrations be quantified by Qubit or other fluorometry-based techniques, as Nanodrop methods tend to overestimate quantity. DNAse treatment during extraction is recommended. For optimal results, the RIN should be at least 7.
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12M reads, 2x150bp
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25M reads, 2x150bp
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50M reads, 2x150bp
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custom RNAseq services
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Full Flow Cell Runs, Illumina
Sample requirements: Prepared and pooled libraries with a final concentration of at least 2nM.
Includes QC of pool (Qubit and TapeStation) and sample sheet generation. PhiX spike-in is optional.
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100cyc, 100M SE or 200M PE reads, 10Gb
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300cyc, 100M SE or 200M PE reads, 30Gb
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600cyc, 100M SE or 200M PE reads, 60Gb
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100cyc, 400M SE or 800M PE reads, 40Gb
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200cyc, 400M SE or 800M PE reads, 80Gb
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FFC.P2; 200cyc, 400M SE or 800M PE reads, 80Gb
| TYPE | Small, DNA, Variant |
|---|
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300cyc, 400M SE or 800M PE reads, 120Gb
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600cyc, 300M SE or 600M PE reads, 180Gb
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50cyc, 1.2B SE or 2.4B PE reads, 60Gb
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100cyc, 1.2B SE or 2.4B PE reads, 120Gb
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200cyc, 1.2B SE or 2.4B PE reads, 240Gb
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300cyc, 1.2B SE or 2.4B PE reads, 360Gb
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Full Flow Cell Runs, Nanopore
Includes a single library prep for your sample or barcoded pool. Both transposase and ligation library prep methods are available.
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10-20Gb output estimated, GridION
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Bioinformatics
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 Variant Calling Standard – ordered via bundle above.
VC Deluxe
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Prokaryotes
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Differential expression
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Differential expression analysis
| TYPE | Small, DNA, Variant |
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Annotate
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