- Start Strong / RNA Extraction
RNA Extraction Services for High-Quality RNA Sequencing
Quality sequencing starts with quality RNA! Let our microbiology experts handle your project and get consistent, sequencing-ready extractions from your precious samples.
RNA Extraction
SeqCoast provides professional RNA extraction services for microbial, microbiome, environmental, and research samples. Our laboratory processes bacteria, fungi, stool, soil, and other challenging matrices using validated bead-beating extraction workflows optimized for downstream RNA sequencing applications, such as differential gene expression analysis.
Why choose SeqCoast RNA extraction services?
Free your team from hundreds of hands-on RNA extractions.
Ensure proper handling to protect precious samples.
Streamline processing for high-volume orders.
Leverage microbiology professionals to drive confident analyses.
RNA extraction applications
Our RNA extraction services can be paired with any of our RNA next-generation sequencing (NGS) workflows!
SeqCoast RNA extraction services support many downstream applications:
- Transcriptomics
- Differential gene expression
- Metatranscriptomics
-
Poly-A enrichment bulk RNAseq
- Eukaryotes only
-
Ribodepletion bulk RNAseq
- Prokaryotes and eukaryotes
- Microbe-host interactions
Mastering complex matrices and microbes
Are your cells difficult to lyse? Does your organism have a tough cell wall? SeqCoast Genomics uses bead beating to extract quality RNA from even the toughest, difficult-to-lyse microbes and matrices.
- Staphylococcus aureus, Bacillus velezensis, Streptococcus pneumoniae
- Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens
- Saccharomyces cerevisiae (yeast), filamentous fungi
- Stool, saliva, skin swabs, lavage fluid
- Soil, sediment, wastewater, algae
- Biofilms, filters, swabs, and custom matrices
Simplify your large-scale projects
Our expertise and high-throughput capabilities make us the ideal partner for large extraction projects, whether you have dozens or tens of thousands of samples.
Let us streamline your extensive sequencing projects by performing RNA extractions for you:
- High-throughput, automated workflows
- Oversight and judgement by microbial genomics experts
- Consistent processing across large studies
- Reduce time spent on logistics by using our CoOrdinated Management and Processing for Accelerated Sequencing Services (COMPASS) system
Beyond standard: tailored solutions for complex needs
Some sample types need more than a standard workflow. For difficult matrices and larger custom programs, our team can develop and validate a tailored extraction approach with you.
Pricing — the more you order, the lower the price!
Service specifications: precision and care in every step
48+ samples
- We offer our COMPASS system for CoOrdinated Management and Processing for Accelerated Sequencing Services
- COMPASS allows for the fastest processing and the lowest price!
- You will receive a custom shipping container with validated plasticware, stabilization buffer, & prepaid shipping label at no additional charge.
24-48 samples
- Must be sent in a 96-well plate.
- 96-well plates should be fully skirted and sealed well with a foil seal (plastic seals are very prone to leaking during shipping)
1-23 samples
- We accept individual 1.5 mL tubes that are well-labeled and organized
- 50-100mg, supernatant removed.
- Dry ice
- Sample must be homogenized and able to be transferred to a new tube using a pipette.
- We will only be able to take forward 200ul of homogenized sample into our extraction workflow.
- Room temperature, on ice packs, or on dry ice
- Submit pre-loaded into bead-beating tubes.
- We recommend MagMax Microbiome Bead Beating Tubes, but any 2ml screw-cap tube with bead beating matrix is acceptable.
- Ship frozen on dry ice for most sample types.
Please send
All samples must be securely packaged in a clean, organized fashion using secondary containment. Leaking samples will be immediately disposed of, as required by our biosafety measures.
Please do not send
Bags of tubes, individual tubes wrapped in parafilm, large-format tubes (e.g. 50ml conicals), non-sterile/soft-sided containers (e.g. plastic bags), unsealed cell culture 96- well plates. We’ve seen it all! Please double-check your shipments to ensure your samples make it to us safe and sound.
Frequently Asked Questions
What sample types can be used for RNA extraction?
We accept bacterial cultures, fungal cultures, stool, soil, sediment, wastewater, swabs, algae samples, filters, and more! Please refer to our Sample Prep Guidelines for more details.
Can SeqCoast perform RNA extraction for RNAseq?
Yes! Our extraction workflows are compatible with any of our downstream RNA sequencing workflows, including mixed-species metatranscriptomics. We use bead-beating to ensure thorough lysis, even for tough-to-lyse microbes.
Do you offer high-throughput RNA extractions?
All the time! SeqCoast regularly processes large-scale projects ranging from a few dozen to thousands of samples using our automated & scalable extraction workflows and our COMPASS project management system.