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Prokaryote Assembly and Annotation
SeqCoast’s end-to-end pipeline transforms raw sequencing data into assembled and annotated genomes, returning comprehensive and reproducible assembly and annotation results.
Assembly & Annotation
What is annotation and assembly?
Bacterial genome assembly and annotation transforms raw sequencing reads into a genome sequence and identifies biologically meaningful genomic features. SeqCoast’s genome assembly and annotation pipeline generates high-quality annotated bacterial genome assemblies, delivering publication-ready results.
Bacterial genome assembly and annotation has applications across fields including:
- Microbial genomics and genome characterization
- Pathogen surveillance and outbreak investigation
- Antimicrobial resistance and virulence gene discovery
- Comparative genomics and bacterial strain characterization
- Evolutionary, phylogenetic, and population genomics studies
- Novel isolate characterization and reference genome generation
- Functional genomics and metabolic pathway analysis
- Comparative analysis of bacterial isolates across treatments, conditions, locations, or timepoints
What's included?
Our report includes accessible, high-quality analysis results ready for publication.
Publication-ready analysis
- Fully reproducible and comprehensive report with interactive visualizations and data.
- Standalone versions of all figures and tables.
- Clear, organized file access instructions.
Documentation of methods and citations
- Method descriptions provided for publication process.
- Software versions maintained and managed in GitHub and Seqera Nextflow.
- Citations provided for publication reference.
Quality control
- Every order is reviewed by an expert bioinformatics scientist to ensure accurate, high-quality results.
- Interactive MultiQC report summarizes sequencing and processing quality metrics.
Analysis process
Our analyses are built with state-of-the-art computational tools and industry-standard quality control tools tailored to our workflow.
- Thorough quality preprocessing and quality control results.
- Reports on potential contamination within samples to enable the highest quality assembly.
- Generates a draft genome assembly.
- Remove artifacts, correct errors, and improve consensus of the assembled reference genome.
- QC analyzed throughout the pipeline including reports on assembly and annotation quality.
Need something beyond the standard workflow?
We understand that some research requires specialized attention. Customized processing, reporting, and bioinformatics analyses are available for specialized research projects.
Pricing — the more you order, the lower the price!
Why order SeqCoast analysis?
Get interpretable results, not just a folder of output files. Transform raw sequencing data into actionable insights.
Save time with analysis infrastructure that's ready to scale from pilot studies to enterprise-scale projects.
Reduce cost and complexity by leveraging our established cloud computing environment and validated workflows.
Accelerate research with rapid turnaround times and high-throughput processing capabilities.
Skip building and maintaining an assembly and annotation pipeline of your own, at any batch size.
Trust your results with rigorous QC, proven pipelines, and expert scientific review.
Data delivery, security, and access
- Our analysis pipelines are managed by bioinformatics experts using modern, version-controlled workflow frameworks that enable standardized, documented, and reproducible analyses.
- We maintain security controls designed to protect customer data throughout the data lifecycle. All data are produced and analyzed on USA-based equipment and AWS cloud infrastructure. Learn more about our world-class informatics processes here.
We make it easy to access your data!
- Data delivery options include SFTP Download, AWS transfer, Box.com, and alternatives on request.
- Data are stored on AWS cloud infrastructure in the United States, providing scalable storage, high availability, and industry-recognized security controls.
Frequently Asked Questions
Does this analysis require long or short reads?
This analysis can be performed with either short or long reads, or can be run on a combination of long and short reads (hybrid sequencing). If you are unsure which option is the best choice for you, get in touch with us to speak to our team of microbiologist experts.