List pricing is provided below, and you can create a quote or place an order directly from this page.
- We offer custom pricing on orders with 24+ samples or a substantial total data volume.
- Contact us to obtain a personalized quote that’s adjusted to your project’s size and timeline.
Price
DNA Extraction
$20/Sample
Short read sequencing, Illumina
Sample requirements: >10ng/ul in at least 30ul of volume.
Our short read sequencing service provides 150bp paired end reads. Short reads can be useful for a range of applications, including genotyping of clones, whole population and metagenomic sequencing, and amplicon/microbiome profiling. Quantities listed are guaranteed minimums.
Price
Qty
Variant Calling
$15/sample
Our variant calling service includes mapping of short reads against a reference genome. Our service builds upon the best-of-class breseq software to provide intuitive insights into the genetic differences between your sample and the genome reference.
DNA Extraction
$15/sample
200 Mbp/1.3 Million reads
From: $0.00
Additional information
Illumina; 200 Mbp/1.3 million reads
| TYPE | Small, DNA, Variant |
|---|
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400 Mbp/2.7 Million reads
From: $0.00
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650 Mbp/4.3 Million reads
From: $0.00
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1 Gbp/6.7 million reads
From: $0.00
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2 Gbp/13.3 million reads
From: $0.00
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Illumina; 2 Gbp/13.3 million reads
| TYPE | Small, DNA, Variant |
|---|
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5 Gbp/33.3 million reads
From: $0.00
Additional information
Illumina; 5 Gbp/33.3 million reads
| TYPE | Small, DNA, Variant |
|---|
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10 Gbp/66.7 million reads
From: $0.00
Additional information
Illumina; 10 Gbp/66.7 million reads
| TYPE | Small, DNA, Variant |
|---|
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25 Gbp/167 million reads
From: $0.00
Additional information
Illumina; 25 Gbp/167 million reads
| TYPE | Small, DNA, Variant |
|---|
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50 Gbp/333 million reads
From: $0.00
Additional information
Illumina; 50 Gbp/333 million reads
| TYPE | Small, DNA, Variant |
|---|
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Long read sequencing, Oxford Nanopore
Sample requirements: >40ng/ul in at least 60ul of volume.
Our long read sequencing service provides reads up to 100kbp (occasionally much longer) in length. To maximize read length we strongly recommend using our extraction service, because long DNA fragments may shear during shipping and with some extraction methods.
Long reads can be useful for a range of applications, including sequencing longer amplicons, repetitive plasmids or viruses, spanning difficult to map/assemble regions, and for resolving complex metagenomes. Quantities listed are guaranteed minimums.
Price
Qty
Variant Calling
$15/sample
DNA Extraction
$15/sample
Nanopore; 300 Mbp
From: $0.00
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Nanopore; 600 Mbp
From: $0.00
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Nanopore; 900 Mbp
From: $0.00
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Nanopore; 1.5 Gbp
From: $0.00
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16S/ITS Amplicon (Illumina or Nanopore)
-sequencing service only
Sample requirements: >10ng/ul in at least 30ul of nuclease-free water, samples must be free of PCR inhibitors including EDTA.
Use full-length 16S or ITS sequencing to maximize species and strain-level resolution.
Pair with our "16S/ITS microbiome analysis" service to get community composition and diversity metrics, in both table and graph formats.
Custom pricing starts at 24 samples; contact us to get a volume-based quote.
Price
Qty
DNA Extraction
$15/sample
V3/V4 20k reads, 2x300bp
From: $0.00
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ITS2 20k reads, 2x300bp
From: $0.00
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V3/V4 50k reads, 2x300bp
From: $0.00
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ITS2 50k reads, 2x300bp
From: $0.00
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V3/V4 100k reads, 2x300bp
From: $0.00
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ITS2 100k reads, 2x300bp
From: $0.00
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16S full-length 10k reads
From: $0.00
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ITS full-length 10k reads
From: $0.00
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16S full-length 25k reads
From: $0.00
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ITS full-length 25k reads
From: $0.00
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16S full-length; 50k reads
From: $0.00
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ITS full-length; 50k reads
From: $0.00
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Consider pairing with our “16S/ITS microbiome analysis” service!
Please contact us for custom primers (including ITS) and for large orders.
Hybrid whole genome sequencing
-bundled extraction and sequencing
Sample requirements: frozen cell pellet (50-100mg) or sealed, pre-grown agar plate.
Our hybrid sequencing service provides both 150bp paired end reads in combination with long reads for a given sample. HMW DNA extraction is also included!
This sequencing strategy is especially useful for building robust reference genomes; the long reads drastically reduce the number of contigs and the depth afforded by the short reads increases the confidence of each nucleotide position.
Price
Qty
Illumina 400 Mbp + Nanopore 300 Mbp (up to 8Mbp genomes)
From: $0.00
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Hybrid; Illumina 1 Gbp + Nanopore 600 Mbp (up to 20Mbp genomes)
$294.00 Add to cart -
Hybrid; Illumina 2 Gbp + Nanopore 1.2 Gbp (up to 40Mbp genomes)
$371.00 Add to cart -
Illumina 1 Gbp + Nanopore 600 Mbp (up to 20Mbp genomes)
From: $0.00 Add to cart -
Illumina 2 Gbp + Nanopore 1.2 Gbp (up to 40Mbp genomes)
From: $0.00 Add to cart
Illumina 1 Gbp + Nanopore 600 Mbp (up to 20Mbp genomes)
From: $0.00
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-
Hybrid; Illumina 2 Gbp + Nanopore 1.2 Gbp (up to 40Mbp genomes)
$371.00 Add to cart -
Illumina 400 Mbp + Nanopore 300 Mbp (up to 8Mbp genomes)
From: $0.00 Add to cart -
Illumina 2 Gbp + Nanopore 1.2 Gbp (up to 40Mbp genomes)
From: $0.00 Add to cart -
Hybrid; Illumina 1 Gbp + Nanopore 600 Mbp (up to 20Mbp genomes)
$294.00 Add to cart
Illumina 2 Gbp + Nanopore 1.2 Gbp (up to 40Mbp genomes)
From: $0.00
Related products
-
Illumina 400 Mbp + Nanopore 300 Mbp (up to 8Mbp genomes)
From: $0.00 Add to cart -
Illumina 1 Gbp + Nanopore 600 Mbp (up to 20Mbp genomes)
From: $0.00 Add to cart -
Hybrid; Illumina 2 Gbp + Nanopore 1.2 Gbp (up to 40Mbp genomes)
$371.00 Add to cart -
Hybrid; Illumina 400 Mbp + Nanopore 300 Mbp (up to 8Mbp genomes)
$245.00 Add to cart
Consider pairing with our “Assembly and annotation, Prokaryotes” service!
Contact us for large orders and custom analysis needs.
Poly-A Enrichment RNAseq, Illumina
-compatible with eukaryotes only
Our RNA sequencing service provides 150bp paired end reads.
Please send >50ng/ul of RNA in a volume of at least 20ul (more is better!), shipped frozen on dry ice.
We recommend that sample concentrations be quantified by Qubit or other fluorometry-based techniques, as Nanodrop methods tend to overestimate quantity. DNAse treatment during extraction is recommended. For optimal results, the RIN should be at least 7.
Price
Qty
Differential Expression
$53/sample
RNA Extraction
$50/sample
polyA; 12M reads, 2x150bp
From: $0.00
Additional information
Differential expression analysis
| TYPE | Small, DNA, Variant |
|---|
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polyA; 25M reads, 2x150bp
From: $0.00
Additional information
Differential expression analysis
| TYPE | Small, DNA, Variant |
|---|
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polyA; 50M reads, 2x150bp
From: $0.00
Additional information
Differential expression analysis
| TYPE | Small, DNA, Variant |
|---|
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polyA; 100M reads, 2x150bp
From: $0.00
Additional information
Differential expression analysis
| TYPE | Small, DNA, Variant |
|---|
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Ribodepletion RNAseq, Illumina
-eukaryotes and prokaryotes
Our RNA sequencing service provides 150bp paired end reads.
Please send >50ng/ul of RNA in a volume of at least 20ul (more is better!), shipped frozen on dry ice.
We recommend that sample concentrations be quantified by Qubit or other fluorometry-based techniques, as Nanodrop methods tend to overestimate quantity. DNAse treatment during extraction is recommended. For optimal results, the RIN should be at least 7.
Price
Qty
Differential Expression
$53/sample
RNA Extraction
$50/sample
ribo; 12M reads, 2x150bp
From: $0.00
Additional information
Differential expression analysis
| TYPE | Small, DNA, Variant |
|---|
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ribo; 25M reads, 2x150bp
From: $0.00
Additional information
Differential expression analysis
| TYPE | Small, DNA, Variant |
|---|
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ribo; 50M reads, 2x150bp
From: $0.00
Additional information
Differential expression analysis
| TYPE | Small, DNA, Variant |
|---|
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ribo; 100M reads, 2x150bp
From: $0.00
Additional information
Differential expression analysis
| TYPE | Small, DNA, Variant |
|---|
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Bioinformatics
Price
Qty
Prokaryotes
From: $0.00
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A-M analysis
From: $0.00
Additional information
Amplicon/microbiome analysis
| TYPE | Small, DNA, Variant |
|---|
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Differential expression
From: $0.00
Additional information
Differential expression analysis
| TYPE | Small, DNA, Variant |
|---|
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BP
From: $0.00
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– including strain typing, outbreak identification, and monitoring
Custom bioinformatics services
– including metagenomics, transcriptome assembly, differential expression, pangenomic analysis
– please contact us for a custom quote
Shipping - return of DNA samples
We will ship DNA samples on ice packs in an insulated, rigid box.
We do not have access to dry ice, however, please be assured that purified dsDNA is a very stable molecule even at room temperature.
– 2 business day delivery
From: $0.00
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– overnight delivery
From: $0.00
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Prepaid Sequencing and Bioinformatics
Price
Qty
Insights Services
From: $0.00
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Have a large project? We offer tailored quotes and attractive discounts on orders of 24+ samples or bulk sequencing orders, such as full flow cells and substantial data volumes.
Contact us to obtain a personalized quote that’s adjusted to your project’s size and timeline.
Sample Preparation Guidelines
Nucleic Acid Samples
| Service | Concentration* | Volume | Buffer | Shipping Considerations |
|---|---|---|---|---|
|
Short read sequencing, Illumina |
>10 ng/µL |
>30 µL |
nuclease-free water or dilute TE (1:10) |
Can be shipped at room temperature. |
|
Long read sequencing, Oxford Nanopore |
>50 ng/µL |
>60 µL |
nuclease-free water |
Should be shipped with ice packs or on dry ice. |
|
†Poly-A Enrichment RNAseq, Illumina |
>25 ng/µL RNA |
>20 µL. Please include a second aliquot (~5 µL) for sample QC. |
nuclease-free water or dilute TE (1:10) |
RNA should be shipped overnight on dry ice. |
|
†Ribodepletion RNAseq, Illumina |
>25 ng/µL RNA |
>20 µL. Please include a second aliquot (~5 µL) for sample QC. |
nuclease-free water or dilute TE (1:10) |
RNA should be shipped overnight on dry ice. |
|
16S Amplicon (Illumina or Nanopore) |
>10 ng/µL |
>30 µL |
nuclease-free water |
Can be shipped at room temperature. |
|
Full Flow Cell Runs, Illumina |
Prepared and pooled libraries with a final concentration of at least 2 nM. |
RSB + Tween |
Can be shipped with ice packs or on dry ice. |
|
|
Full Flow Cell Runs, Nanopore |
Same as long read Nanopore |
nuclease-free water |
Should be shipped with ice packs or on dry ice. |
|
*We recommend quantifying by Qubit, as Nanodrop can overestimate concentrations >10-fold if contaminants are present.
We highly recommend eluting samples in nuclease-free water or diluted TE, as excessive EDTA can interfere with with library prep. Extraction contaminants (salts, phenol, polysaccharides, etc.) can cause sample failure.
†Note for RNA services: To minimize the amount of time that your RNA samples are thawed, please label your tubes with their SeqCoast tube IDs and dilute your samples to <250 ng/µL prior to shipping.
Services that include DNA Extraction
| Service | Sample Type | Shipping Considerations |
|---|---|---|
|
Hybrid whole genome sequencing, Illumina/Nanopore OR Strain typing, Illumina |
Frozen cell pellets (50 - 100 mg), all supernatent removed; OR Sealed, pre-grown agar plates - must have enough growth to allow us to easily collect 50 - 100 mg of colonies.* |
Ship frozen cell pellets on dry ice. Ship agar plates with ice packs or at room temperature. |
*We have found that ~10e9 E. coli cells is sufficient for our extraction workflows.