Full Flow Cell Services
Sequencing and bioinformatics from genomics experts
Short Read Full Flow Cells - Illumina
| Flow Cell Type | Read Qty. | Read Length | Cycle Qty. | Data Volume |
|---|---|---|---|---|
|
P1; Illumina short read |
100M single-end or 200M paired-end |
100 bp |
100 cycles |
10 Gb |
|
300 bp |
300 cycles |
30 Gb |
||
|
600 bp |
600 cycles |
60 Gb |
||
|
P2; Illumina short read |
400M single-end or 800M paired-end |
100 bp |
100 cycles |
40 Gb |
|
200 bp |
200 cycles |
80 Gb |
||
|
300 bp |
300 cycles |
120 Gb |
||
|
P2; Illumina short read |
300M single-end or 600M paired-end |
600 bp |
600 cycles |
180 Gb |
|
P3; Illumina short read |
1.2B single-end or 2.4B paired-end |
50 bp |
50 cycles |
60 Gb |
|
100 bp |
100 cycles |
120 Gb |
||
|
200 bp |
200 cycles |
240 Gb |
||
|
300 bp |
300 cycles |
360 Gb |
||
Submission Requirements
- Read configuration desired
- e.g. 2x150bp paired end reads
- Read Lengths listed here represent the maximum read length for each configuration. This can be customized if you need a specific read length!
- Multiplexing strategy
- List of indexes used, unique dual vs combinatorial, etc.
- Whether PhiX spike-in is needed
- We recommend 35-40% PhiX for low-diversity library pools
- Confirm that custom sequencing primers are not needed for the NextSeq2000
Service Includes
- QC of your sample via Qubit and Tapestation
- Dilution to loading concentration
- Demultiplexing analysis using the customer provided data sheet
- Data delivery within 14 business days
- Complete Illumina sequencing output with sequencing stats and summary
- Fastq files for individual samples according to customer-provided demultiplexing key
- Materials and methods summary
Sample Requirements
- Prepared and pooled libraries with a final concentration of at least 2 nM
Optional Add-Ons
- DNA extraction
- Library prep
- Library pooling
Long Read Full Flow Cells - Nanopore
| Flow Cell Type | Read Length | Data Volume |
|---|---|---|
|
GridION |
Read length is highly variable and dependent on high quality sample input. We offer DNA extraction services using workflows designed to maximize fragment length and quality from diverse sample types. |
Typical output between 10-20 Gbp |
|
PromethION |
Typical output between 60-120 Gbp |
Submission Requirements
- Whether native ligation or rapid transposase PCR-free library prep should be used
Service Includes
- QC of your sample via Qubit and Tapestation
- PCR-free library prep for one sample
- Native ligation or rapid transposase
- Data delivery within 14 business days
- One fastq file per sample
- Fast5 files with kinetic data
- Sequencing stats and summary
- Materials and methods summary
Sample Requirements
- Total sample volume of >60 µL at a concentration of at least 50 ng/µL
Optional Add-Ons
- DNA extraction
- Additional library preps
- Adaptive sampling methods to enrich coverage in regions of interest
Contact us to get a custom quote for full flow cells.
Not sure where to start? Our consultation services provide expert advice tailored to your project’s needs.
